4-HO-MiPT

4-HO-MiPT (4-hydroxy-N-methyl-N-isopropyltryptamine) is a synthetic tryptamine belonging to the 4-hydroxy–substituted indole family. First prepared and discussed in the late 1970s, it is structurally related to naturally occurring 4-hydroxy tryptamines found in certain fungi and to other synthetic analogs such as 4-HO-MET and 4-AcO-DMT.

Structurally, 4-HO-MiPT is the N-methyl-N-isopropyl homolog of psilocin: the indole-ethylamine core is retained, while the terminal dimethylamino group is replaced by a methyl–isopropyl substitution. This modification provides a useful contrast within series-based studies of synthetic tryptamines, where small changes in N-substitution can be correlated with differences in chromatographic behavior, ionization efficiency, and fragmentation patterns.

In analytical and forensic laboratories, 4-HO-MiPT is typically included in comparative panels focusing on:

• LC-MS(/MS) and GC-MS fragmentation behavior across 4-hydroxy and 4-alkoxy tryptamine series;
• Chromatographic retention and separation under varied stationary phases and mobile-phase conditions;
• Stability and degradation (for example, oxidation or hydrolysis) under different storage, pH, and sample-preparation conditions.